Water-Soluble Complexes of Methylpyro-Phaeophorbide-а with Carrier Proteins for Photodinamic Therapy
https://doi.org/10.37174/2587-7593-2018-1-1-75-79
Abstract
Practical application of photosensitizers for photodynamic therapy of tumors is limited with insufficient solubility in water and hepatic tropism of tetrapyrrole dyes. The work pursued study of possibility of using a model photosensitizer methylpyro-phaeophorbide α bearing a free carboxylic moiety (MPPPa) in complexes with artificial proteins derived from human alpha-fetoprotein ApE1 and ApE2. Properties of ApE1-MPPPa and ApE2-MPPPa complexes were compared with properties of MPPPa complex with serum albumin. A bathochromic shift of fluorescence maximum of MPPPa in complexes with ApE1 and ApE2 was found in comparison with free MPPPa. This shift gives an evidence of a tight contact between the dye and aromatic amino acids in the carrier proteins. This shift allows expecting a high photochemical activity in the complex photosensitizer in vivo. A high stability of non-covalent ApE1-MPPPa and ApE2-MPPPa complexes substantially exceeding stability of MPPPa complex with serum albumin was demonstrated. Such stability allows preparative purification of these non-covalent complexes with dialysis and/or chromatography.
About the Authors
N. V. Pozdniakova
N.N. Blokhin National Medical Research Center of Oncology
Russian Federation
Moscow
Russian Federation
Moscow
D. V. Belykh
Institute of Chemistry of the Komi Scientific Center, Urals Branch of RAS
Russian Federation
Syktyvkar
Russian Federation
Syktyvkar
I. S. Khudyaeva
Institute of Chemistry of the Komi Scientific Center, Urals Branch of RAS
Russian Federation
Syktyvkar
Russian Federation
Syktyvkar
A. B. Shevelev
N.M. Emanuel Institute of Biochemical Physics of RAS
Russian Federation
Moscow
Russian Federation
Moscow
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Review
For citations:
Pozdniakova N.V., Belykh D.V., Khudyaeva I.S., Shevelev A.B. Water-Soluble Complexes of Methylpyro-Phaeophorbide-а with Carrier Proteins for Photodinamic Therapy. Journal of oncology: diagnostic radiology and radiotherapy. 2018;1(1):75-79. (In Russ.) https://doi.org/10.37174/2587-7593-2018-1-1-75-79
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